Surveyor Substrates: Energy-Transfer Gauges of Active Center Topography during Catalysis
نویسندگان
چکیده
منابع مشابه
Surveyor substrates: energy-transfer gauges of active center topography during catalysis.
An approach is described for the simultaneous measurement of catalysis, the distance from an active-site moiety to fluorescent substrate moieties of an enzyme substrate complex, and the microenvironment of such substrate groups. It is illustrated by means of a three-component energy transfer relay system, consisting of cobalt carboxypeptidase, its fluorescent dansylated peptide substrates, and ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1970
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.67.3.1383